Trichloroethylene(TCE),an important volatile organic solvent,causes a series of toxic damage to human.Conventional genetic mechanisms cannot fully explain its toxicity and carcinogenicity,indicative of the possible involvement of epigenetic mechanisms.Our study was intended to investigate the epigenetic toxicity and underlying mechanisms of TCE.Data showed that 0.3 mM TCE treatment for 24 h increased the growth of L-02 cells transiently.In contrast,subacute exposure to TCE inhibited cell growth and induced the genomic DNA hypomethylation and histone hyperacetylation.Further studies have revealed the TCE-induced DNA hypomethylation in the promoter regions of tumor-related genes,N-Ras,c-Jun,c-Myc,c-Fos and IGF-Ⅱ,promoting their protein levels in a time-dependent manner.These results reveal there is a negative relationship existing between DNA hypomethylation and protein expression in tumor-related gene after TCE exposure under specific epigenetic microenvironment,serving as early biomarkers for TCE-associated diseases.
Trichloroethylene N-Ras c-Jun c-Myc c-Fos IGF-Ⅱ
Fan Wu Caiyun Lai Yan Wang Wei Wang Yueqi Li Gaoqiang Zhang Jianji Gao Zhiliang Zhu Jianhui Yuan Jianping Yang Wenjuan Zhang
Department of Public Health and Preventive Medicine,School of Medicine,Jinan University,Guangzhou,Gu Department of Occupational Health and Occupational Diseases,College of Public Health,Zhengzhou Unive Baoan District Center for Disease Control and Prevention,Shenzhen,Guangdong 518101,P.R.China Nanshan District Center for Disease Control and Prevention,Shenzhen,Guangdong 518054,P.R.China Occupational Health Monitoring Department of The Sixth People's Hospital of Dongguan City,Dongguan G