Acupuncture Reduces Apoptosis of Granulosa Cells in Rats with Premature Ovarian Failure Via Restoring the PI3K/Akt Signaling Pathway
Acupuncture is widely recognized as an effective therapy for premature ovarian failure(POF)in clinical,but information about its potential mechanisms is rarely explored.To investigate the mechanism,fifty SD female rats were randomly divided into normal group,POF group,POF+estradiol-valerate group(abbreviated as estradiol group),and POF+acupuncture group(abbreviated as acupuncture group).The estrous cycle of the rats was tracked by vaginal smears.Their ovaries morphology was observed by hematoxylin-eosin staining.The apoptotic level of granulosa cells was detected by in situ TUNEL fluorescence staining assay.Serum follicle-stimulating hormone(FSH)and estrogen(E2)levels were measured by enzyme-linked-immunosorbent-assay(ELISA).Protein and gene expression of PI3K,Akt,bcl-2,and bax were detected by Western blotting and qPCR.In the acupuncture and estradiol groups,compared with the POF group as controls,the apoptosis number of granulosa cells was significantly decreased(p<0.05).FSH levels were decreased,while E2 levels were increased(p>0.05).The gene and protein expression levels of PI3K,Akt,and bcl-2 were increased,while the expression levels of bax were decreased(p<0.05),and the protein expression level of p-Akt increased.There was no significant difference between the acupuncture group and the estradiol group(p>0.05).Acupuncture was able to regulate hormone levels in POF rats,up-regulate PI3K/Akt signaling pathway,and reduce the apoptosis of granulosa cells.This may be one of the mechanisms of acupuncture treating premature ovarian failure.
PI3K/Akt premature ovarian failure acupuncture granulosa cells
Shiqi Wang Shujun Lin Mingmin Zhu Chenglu Li Shulian Chen Liu Pu Jihuan Lin Luxi Cao Yimin Zhang
Department of Acupuncture,School of Traditional Chinese Medicine,Jinan University,Guangzhou 510632,C Formula-pattern Research Center,School of Traditional Chinese Medicine,Jinan University,Guangzhou 51
国际会议
第三届暨南大学粤港澳大湾区医学与健康发展分论坛——医学研究生学术论坛
广州
英文
229-241
2020-11-28（万方平台首次上网日期，不代表论文的发表时间）