Cichorium intybus L.Promotes Intestinal Uric Acid Excretion by Modulating ABCG2 in Experimental Hyperuricemia
Background Excessive production and/or reduced excretion of uric acid could lead to hyperuricemia,which could be a major cause of disability.Hyperuricemia has received increasing attention in the last few decades due to its global prevalence.Cichorium intybus L.,commonly known as chicory,is a perennial herb of the asteraceae family.It was previously shown to exert potent hypouricemic effects linked with decreasing uric acid formation in the liver by downregulating the activity of xanthine oxidase,and increasing uric acid excretion by upregulating the renal OAT3 mRNA expression.The present study aimed to evaluate its extrarenal excretion and possible molecular mechanism underlying the transporter responsible for intestinal uric acid excretion in vivo.Methods Chicory was administered intragastrically to hyperuricemic rats induced by drinking 10% fructose water.The uricosuric effect was evaluated by determining the serum uric acid level as well as the intestinal uric acid excretion by HPLC.The location and expression levels of ATP-binding cassette transporter,sub-family G,member 2 (ABCG2) in jejunum and ileum were analyzed.Results The administration of chicory decreased the serum uric acid level significantly and increased the intestinal uric acid excretion obviously in hyperuricemic rats induced by 10% fructose drinking.Staining showed that ABCG2 was expressed in the apical membrane of the epithelium and glands of the jejunum and ileum in rats.Further examination showed that chicory enhanced the mRNA and protein expressions of ABCG2 markedly in a dose-dependent manner in jejunum and ileum.Conclusion These findings indicate that chicory increases uric acid excretion by intestines,which may be related to the stimulation of intestinal uric acid excretion via up-regulating the mRNA and protein expressions of ABCG2.
cichorium intybus L. chicory hyperuricemia intestinal uric acid excretion ABCG2
Yu Wang Zhijian Lin Anzheng Nie Meng Bian Bing Zhang
Beijing University of Chinese Medicine, Beijing 100029, China
国际会议
北京
英文
160-171
2019-03-30（万方平台首次上网日期，不代表论文的发表时间）