Biomonitoring for traditional herbal medicinal products using DNA metabarcoding and single molecule, real-time sequencing
Global concerns have been paid to the potential hazard of traditional herbal medicinal products(THMPs).Substandard and counterfeit THMPs,including traditional Chinese patent medicine,health foods,dietary supplements,etc.are potential threats to public health.Recent marketplace studies using DNA barcoding have determined that the current quality control methods are not sufficient for ensuring the presence of authentic herbal ingredients and detection of contaminants/adulterants.An efficient biomonitoring method for THMPs is of great needed.Herein,metabarcoding and single-molecule,realtime (SMRT) sequencing were used to detect the multiple ingredients in Jiuwei Qianghuo Wan(JWQHW),a classical herbal prescription widely used in China for the last 800 years.Reference experimental mixtures and commercial JWQHW products from the marketplace were used to confirm the method.Successful SMRT sequencing results recovered 5416 and 4342 circular-consensus sequencing (CCS) reads belonging to the ITS2 and psbA-trnH regions.The results suggest that with the combination.
Asthma IgE FcεR1-/-Hypotension
Tianyi Xin Zhichao Xu Jing Jia Christine Leon Songnian Hu Yulin Lin Subramanyam Ragupathy Jingyuan Song Steven G.Newmaster
Key Lab of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medi Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3AB, UK Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101,China NHP Research Alliance, Biodiversity Institute of Ontario (BIO), University of Guelph, Guelph N1G 2W1
国际会议
北京
英文
181-194
2019-03-30（万方平台首次上网日期，不代表论文的发表时间）