Inhibition of hydrolytic acidification and enrichment of antibiotic resistome in the microbial communities of mesophilic anaerobic digestion under high oxytetracycline stress
The purpose of this study was to evaluate the impacts of oxytetracycline (OTC) on the development of antibiotic resistance genes in the microbial communities and the performance of mesophilic anaerobic digestion (AD).A mesophilic completely stirred tank reactor operated with continuous OTC feeding enabled to interpret the impacts of OTC.The results showed that methane metabolism of mesophilic AD was inhibited when the OTC concentration was accumulated to around 18,000 mg kg-1,which should due to the decrease in the activities of hydrolytic-fermentative bacteria in the anareobic microbial communities.Besides inhibiting microbial metabolism,accumulation of sludge resistome with the increase of OTC dose was also observed by high-throughput quantitative PCR.Tetracycline resistance genes were the most dominant type (38.47-43.76 %) in the resistome,and tetG,tetX,tetM,tetR,tetQ,tetO and tetL were the dominant resistant subtypes,whose abundances significantly increased when the OTC dose was 40 to 200 mg·L-1.Together with the hike of resistome,integrons and tmnsposons were also enriched facing increasing levels of OTC,indicating horizontal gene transfer might play an important role in the enrichment of resistome.
hydrolytic acidification resistome high-throughput quantitative PCR mesophilic anaerobic digestion oxytetracycline
Z.Tian Y.Zhang M.Yang
State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China
国际会议
The 15th IWA World Conference on Anaerobic Digestion( 第15届IWA世界厌氧大会)
北京
英文
45-48
2017-10-17（万方平台首次上网日期，不代表论文的发表时间）