The active microbial community more accurately reflects the anaerobic digestion process: 16S rRNA (gene) sequencing as a predictive tool
Amplicon sequencing methods targeting the 16S rRNA gene have been used extensively to investigate microbial community composition and dynamics in anaerobic digestion.These methods successfully characterise amplicons,but do not distinguish micro-organisms that are actually responsible for the process.In this research,the archaeal and bacterial community of 48 full-scale anaerobic digestion plants was evaluated on DNA (total community) and RNA (active community) level via 16S rRNA (gene) amplicon sequencing.A significantly higher diversity on DNA compared with the RNA level was observed for archaea,but not for bacteria.Beta diversity analysis showed a significant difference in community composition between the DNA and RNA of both bacteria and archaea.This related with 25.5 and 42.3% of total OTUs for bacteria and archaea,respectively,that showed a significant difference in their DNA and RNA profiles.Similar operational parameters affected the bacterial and archaeal community,yet,the differentiating effect between DNA and RNA was much stronger for archaea.In conclusion,a clear difference in active (RNA) and total (DNA) community profiles was observed,implying the need for a combined approach to estimate microbial community stability in anaerobic digestion.
Biogas Illumina sequencing methane methanogenesis microbiome
Jo De Vrieze Ameet J.Pinto William T.Sloan Nico Boon Umer Z.Ijaz
Center for Microbial Ecology and Technology (CMET), Ghent University, Coupure Links 653, B-9000 Gent Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA Infrastructure and Environment Research Division, School of Engineering, University of Glasgow, UK Center for Microbial Ecology and Technology (CMET), Ghent University, Coupure Links 653, B-9000 Gent
国际会议
The 15th IWA World Conference on Anaerobic Digestion( 第15届IWA世界厌氧大会)
北京
英文
57-60
2017-10-17（万方平台首次上网日期，不代表论文的发表时间）