Characterization of the microbial communities in biogas reactor fed with single substrates stepwise changed to more simplified compounds
Although anaerobic digestion is a widely spread technology,the microbes that mediate the process are still far to be completely characterised.Microbes involved in each step of degradation process are difficult to be identified due to the complex interactions and functional redundancy of the consortium.In this study,extremely specialized methanogenic microbial communities were shaped by stepwise simplified synthetic substrate,namely polysaccharide,monosaccharide,volatile fatty acids mixture,and acetic acid.A stable methane production for each substrate was achieved and the corresponding mierobiomes were characterized using high throughput shotgun sequencing.The results showed that the composition of microbial community changed significantly with different substrates and the metabolic roles of microbes were inferred according to the community dynamics.Clostridium thermocellum was identified as the main hydrolytic bacteria during polysaccharide degradation and it was nearly absent when the reactors were fed with more simple substrates.A syntrophic activity was observed between Coprothermobacter proteolyticus and Methanoculleus sp.When the feed was saccharides and fatty acids but not when acetic acid was the feed.Furthermore,new fatty acid oxidizers were proposed including Anaerobaculum spp.,Subdoligranulum sp.,Parabacteroides sp.and Wolinella succinogenes.Hydrogenotrophic was proven to be the main methanogenesis pathway during saccharide degradation and acetoclastic pathways started to emerge after acetic acid was fed as substrate.
Anaerobic digestion metagenomic analysis stepwise simplified substrate functional roles
X.Zhu P.G.Kougias L.Treu S.Campanaro I.Angelidaki
Department of Environmental Engineering, Technical University of Denmark, Kgs.Lyngby, 2800, DK Department of Biology, University of Padua, Via U.Bassi 58/b, 35131 Padova, Italy
国际会议
The 15th IWA World Conference on Anaerobic Digestion( 第15届IWA世界厌氧大会)
北京
英文
70-73
2017-10-17（万方平台首次上网日期，不代表论文的发表时间）