Time course analysis of bamA and mcrA gene expression and microbial activity during batch anaerobic digestion of phenol and p-cresol
Previous work from our lab showed an abundance increase of members belonging to the order Methanobacteriales,the genus Syntrophorhabdus and the functional gene bamA after successive anaerobic degradation of phenolic compounds in batch mode.Based on this,the aim of the present work was to answer the question if there exists a correlation between the activity of these microorganisms and functional genes with the kinetics of anaerobic digestion process of these compounds,in order to establish the potential use of these biological variables as indicators of process stability.To achieve this objective,anaerobic digestion of a mixture of phenol and p-cresol (220 and 100 mg.L-1,respectively) were conducted in batch reactors where five sludge samples were obtained during the course of the reaction.RT-qPCR for bamA,mcrA,Methanobacteriales and Syntrophorhabdus were made and Pearson correlation tests were performed to establish the relationships between transcripts levels of functional genes,activity of microorganisms and degradation rate ofphenolics together with methane production.Results showed that transcripts of functional genes and activity levels of Syntrophorhabdus and Methanobacteriales are highly dynamic during the batch anaerobic digestion of phenol and p-cresol.No relationship was found either between kinetics of the process and mcrA,Syntrophorhabdus or Methanobacteriales activity.On the other hand,bamA expression correlated positively with phenolics concentration and degradation rate (p < 0.05) indicating that transcription level of this gene is regulated by phenolic concentration and even more interesting,the expression level measurement of this gene could serve as a potential tool for monitoring the degradation capacity in anaerobic digesters treating this kind of compounds.
Functional genes phenolic compounds bamA mcrA RT-qPCR
O.Franchi P.Bovio F.Rosenkranz R.Chamy
Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2085 Laboratorio de Ecología Microbiana, Departamento de Bioquímica y Genómica Microbiana.Instituto de In Núcleo Biotecnología Curauma, Pontificia Universidad Católica de Valparaíso, Avenida Universidad 330 Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2085
国际会议
The 15th IWA World Conference on Anaerobic Digestion( 第15届IWA世界厌氧大会)
北京
英文
78-83
2017-10-17（万方平台首次上网日期，不代表论文的发表时间）