MiDAS 2.0: The site-specific curated database of microorganisms in activated sludge and anaerobic digesters
An understanding of the microbial communities and dynamics in wastewater treatment systems is a powerful tool for process optimization and design.The advent ofamplicon sequencing of the 16S rRNA gene now allows the diversity within the microbial communities to be sampled sufficiently to describe the composition and dynamics of the most abundant organisms.However,to understand the relationship between the population dynamics and operational parameters of the system,a functional role must be attributed to each organism.The Microbial Database for Activated Sludge (MiDAS) and Anaerobic Digesters (AD) provides a curated taxonomy for abundant and important microorganisms in these systems and in the incoming wastewater and integrates it into a community knowledge web platform about the microbes present therein.The MiDAS taxonomy proposes putative names for all the abundant genus-level microorganisms in AS and AD systems.The online database covers >250 genera found in biological nutrient removal treatment plants,based on extensive in-house surveys with 16S rRNA gene amplicon sequencing,including full-scale AS (20 plants,8 years) and AD systems (36 reactors,18 plants,4 years).Surveys also include the Archaea.The MiDAS field guide is available as a web resource (www.midasfieldguide.org).The website provides a searchable database of referenced information about each abundant and/or important genus present in the influent,AS and AD systems acting as a central,on-line repository for current knowledge about activated sludge organisms.Information includes morphology (e.g.filamentous),metabolic guilds relevant to the AS and AD environments (e.g.polyphosphate accumulating organisms,ammonia oxidizing bacteria,methanogens),abundance information and many others.The MiDAS field guide is intended as a collaborative platform for researchers,consultants and wastewater treatment practitioners that link names of important microorganisms in AS and AD systems to the wealth of present and future functional information about their ecology.
Activated sludge anaerobic digester MiDAS phylogeny
M.Nierychlo S.J.McIlroy R.H.Kirkegaard B.McIlroy M.Stokholm-Bjerregaard S.M.Karst M.Albertsen P.H.Nielsen
Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark
国际会议
The 15th IWA World Conference on Anaerobic Digestion( 第15届IWA世界厌氧大会)
北京
英文
107-110
2017-10-17（万方平台首次上网日期，不代表论文的发表时间）