Cold shocks of anammox biofilm stimulate nitrogen removal at 10 ℃
The adaptation of anammox to low temperatures (10-15 ℃) is important for sustaining energy-efficient nitrogen removal from wastewater in the main stream of municipal WWTP.But,current adaptation methods are based on the stepwise reduction of operational temperature or enrichment from activated sludge,which both take months or even years.To reduce the time necessary to adapt anammox to low temperatures,this study describes a new approach: exposing anammox microorganisms to an abrupt temporary reduction of temperature described as cold shock.Anammox biomass in a moving bed biofilm reactor was subjected to three consecutive cold shocks (reduction from 24 to 5 ℃),each taking eight hours.Control was operated without the shocks.After each shock,activity of anammox microorganisms was determined ex situ using batch assays at low temperatures (10,13,16,and 20 ℃).Cold shocks stimulated the activity of anammox at 10 ℃ to 0.054 kg-N kg-VSS-1 d-1 after the third shock,which is similar to the highest activities obtained for cold-enriched or adapted anammox reported in the literature.FISH analysis showed that the cold shock effect did not deteriorate the abundance of any detected anammox bacteria with Ca.Brocadia fulgida being the dominant species.Although we only report short-term adaptation and understanding the mechanism needs further assessment,this study reports.
main stream anammox low temperature adaptation nitrogen removal
V.Kouba R.Darmal D.Vejmelkova P.Jenicek J.Bartacek
University of Chemistry and Technology Prague, Department of Water Technology and Environmental Engi ETH Zürich, Department of Chemistry and Applied Biosciences, Vladimir-Prelog-Weg 1-5/10, 8093 Zürich
国际会议
The 15th IWA World Conference on Anaerobic Digestion( 第15届IWA世界厌氧大会)
北京
英文
199-202
2017-10-17（万方平台首次上网日期，不代表论文的发表时间）