Thermophilic biohydrogen and bioelectricity production for energy recovery from xylose
In this study,thermophilic biohydrogen (55-70℃) and bioelectricity (55℃) production from xylose were compared with the corresponding mesophilic (37℃) processes,focusing on both process efficiency and microbial community composition.Biohydrogen production was studied both in batch and continuous mode by using serum bottles and fluidised bed reactors (FBRs),respectively.Bioelectricity production was evaluated in two-chamber microbial fuel cells (MFCs) operated in fed-batch mode.The highest hydrogen (H2) yield achieved was 1.85 mol H2 mol-1 xyloseconsumed in batch cultures at 55℃.A higher average H2 yield was achieved in the therrnophilic than in the mesophilic FBR,with a maximum yield of 1.2 mol H2 mol-1 xyloseconsumed at both 55 and 70℃.Thermoanaerobacter was the predominant species in the thermophilic FBR,regardless of the temperature.In the MFCs,about 10-times higher power density was generated under mesophilic than thermophilic conditions.However,a gradual decrease towards more negative anode potential in the thermophilic MFC suggested the enrichment of a thermophilic exoelectrogenic community.Temperature affected significantly both microbial diversity and activity,which were determined by high-throughput sequencing of 16S rRNA genes (DNA) and of 16S rRNA (cDNA),respectively.In particular,a significant difference was observed in the structure of the microbial community collected from three different sampling points of the same MFC (anodic biofilm,anode solution and membrane).Known exoelectrogenic bacteria (mainly in the anodic biofilms),and species associated with metabolic pathways other than bioelectricity generation,such as H2 oxidation and methanogenesis,were detected.Methanogens,which were found only in the thermophilic MFC,may have competed for substrate with exoelectrogenic microorganisms,resulting in the low power density observed.Aerobic microorganisms found on the membrane may play a role in preventing oxygen intrusion from the cathodic chamber to the anode side.
Bioelectricity biohydrogen exoelectrogenic bacteria MiSeq Thermoanaerobacter Thermophilic
Paolo Dessì Aino-Maija Lakaniemi Estefania Porca Johanna Haavisto Gavin Collins Piet N.L.Lens
Department of Chemistry and Bioengineering, Tampere University of Technology, P.O.Box 541, FI-33101 Microbial Communities Laboratory, School of Natural Sciences, National University of Ireland Galway,
国际会议
The 15th IWA World Conference on Anaerobic Digestion( 第15届IWA世界厌氧大会)
北京
英文
235-238
2017-10-17（万方平台首次上网日期，不代表论文的发表时间）