Electron bifurcation mechanism and homoacetogenesis explain products yields in mixed culture anaerobic fermentations
Anaerobic fermentation of wastes with mixed cultures is a promising avenue to treat residues while producing addedvalue products.But thus far,the lack of control and prediction of the microbial community behaviour hinders any industrial application.One of the fundamental limitations of the process,is that we are not able to foresee the outcome of the process as its stoichiometry is not fully defined.In this work,we present a new metabolic network of fermentation of glucose by mixed cultures.The metabolic network that defines the stoichiometry of the process includes for the first time Electron Bifurcation and Homoacetogenesis.This novel metabolic network was successfully confronted with experimental data through NADH (i.e.electron) balances.Results show that the inclusion of Electron Bifurcation in the network provides a better description of experimental results and suggest its occurrence in the process.Homoacetogenesis,together with Electron Bifurcation,helps to correctly predict the H2 and CO2 gaseous yields.The new stoichiometry proposed accurately predicts the product yielding,including gaseous products,observed in glucose anaerobic fermentations.This supports the consideration of Electron Bifurcation as an important biochemical mechanism in microbial mixed cultures fermentations and remarks the importance of including Homoacetogenesis when analysing anaerobic fermentations.
Anaerobic fermentation metabolic network electron bifurcation homoacetogenesis VFA production
A.Regueira R.González-Cabaleiro I.D.Ofi(t)eru J.Rodríguez J.M.Lema
Dep.of Chemical Engineering, Institute of Technology.Universidade de Santiago de Compostela, RConst School of Chemical Engineering and Advanced Materials, Merz Court, Newcastle Upon Tyne, NE1 7RU, Uni Dep.Chemical and Environmental Engineering (CEE) Masdar Institute of Science and Technology.PO Box 5 Dep.of Chemical Engineering, Institute of Technology.Universidade de Santiago de Compostela, RConst
国际会议
The 15th IWA World Conference on Anaerobic Digestion( 第15届IWA世界厌氧大会)
北京
英文
389-392
2017-10-17（万方平台首次上网日期，不代表论文的发表时间）