Identification of metabolic pathways in a continuously operated methanogenic reactor through δ13C analysis: A simulation study
An anaerobic digestion model with distinction of 12C and 13C isotopes and including acetogenesis,acetate oxidation,acetoclastic and hydrogenotrophic methanogenesis processes was used to quantify the dynamic variations of the methanogenic and acetate uptake pathways.Isotopic effects were considered for the biological processes,acid-base and liquid-gas equilibria.The simulations with data of a mesophilic upflow anaerobic filter indicated contributions of acetate oxidation,fAO,between 0.12 and 0.15.Simulation with organic loading rates (OLR) above 5.9 gCOD L-1 d-1 resulted in an increase of the hydrogenotrophic methanogenesis contribution,fMC,up to 0.54 in contrast to contributions of 0.30 to 0.34 at lower OLRs.The quantification of these metabolic routes are in line with previous studies using calculation based on 13C biological fractionation and with 13C labeled acetate.Hence,the 13C modeling approach has potential to be used for parameter identification,as biomass growth kinetics are integrated within the simulation of isotopic effects.However,this proposed model structure results in a more laborious model implementation and requires a larger amount of 13C data than previously published models,especially for biogas plants with heterogeneous substrates.
Stable carbon isotopes fractionation ADM1 syntrophic acetate oxidation.
Manfred Lübken Tito Gehring Marc Wichern
Institute of Urban Water Management and Environmental Engineering, Ruhr-Universit(a)t Bochum, Universit(a)tsstrasse 150, 44780 Bochum, Germany
国际会议
The 15th IWA World Conference on Anaerobic Digestion( 第15届IWA世界厌氧大会)
北京
英文
407-410
2017-10-17（万方平台首次上网日期，不代表论文的发表时间）