Substrate induced response in the biogas microbial community relates back to inoculum source
In this study,the effect of adding wheat husk as a co substrate during co-digestion of grass-manure was investigated.The reactors were initially started with two different inoculums and were operated with the same grass-manure mixture for more than 3 HRT prior to the present study.The initial inoculums differed in the microbial community structure and the degradation efficiency of straw/cellulose but during operation with the same substrate (manure/grass) they were unified regarding both performance and microbial community,analysed by illumina sequencing targeting 16S rRNA.However,looking at the potential cellulose degrading community targeting glycoside hydrolase families 5 and 48 a difference was seen depending on the initial inoculum.The aim of the present study was to further investigate the importance of the initial inoculum source for biogas production from a fibre rich material when a change in the substrate composition was made.Our hypothesis was that the initial inoculum source would have impact on the process performance and community structure in spite of being similar at the time point for the start-up of this experiment.Wheat husk was selected as a co-substrate and added all at once or at two occasions every day.The process performance was evaluated by monitoring chemical parameters and by analysing the microbial community in the same way as in the previous experiment.In addition,the methane potential of the substrates was evaluated in BMP tests initiated with inoculums taken from the reactors before and after operation with husk.The results revealed similar process performance independent of feeding schedule and inoculum sources.Addition of the husk increased the volumetric methane production but resulted in a decrease in the specific methane production,possibly explained by a decrease in the species richness of the microbial community.In spite of overall similar performance,the community diverged depending on the initial inoculum source,i.e.the repose in the microbial community due to the husk related back to the initial inoculum.The feeding strategy had an impact of the development of the cellulose degrading community as well as on the final methane potential of cellulose,which was slightly higher when the wheat husk was added simultaneously with the grass/manure mixture.
Anaerobic digestion Performance of biogas production CSTR BMP-test illumina sequencing T-RFLP glycoside hydrolase families 5 and 48
Tong Liu Li Sun (A)ke Nordberg Anna Schnürer
Department of Molecular Science, Swedish University of Agricultural Sciences, Uppsala, Sweden Department of Energy and Technology, Swedish University of Agricultural Sciences, Uppsala, Sweden
国际会议
The 15th IWA World Conference on Anaerobic Digestion( 第15届IWA世界厌氧大会)
北京
英文
437-441
2017-10-17（万方平台首次上网日期，不代表论文的发表时间）