Co-inoculation of cellulolytic rumen bacteria with methanogenic sludge to enhance methanogenesis of rice straw
Ruminal microbiota co-inoculated with methanogenic sludge were applied in a 2 L anaerobic system.The experiment lasted 48 days in three stages with different substrate loading rates of 3 g d-1,7 g d-1 and 14 g d-1.The fermentation performance and microbial characteristics were assessed.The mean methane yields of different stages increased 1.79-,2.07-and 2.26-fold compared with control samples,while the methane content of biogas varied from 32 to 44%,significantly different (P<0.05) from the control reactor.There was no corresponding accumulation of volatile fatty acids (VFAs) during the whole anaerobic digestion,indicating system stability.The cellulolytic activities improved significantly (P<0.05) compared with control samples.Co-inoculation not only added methanogens but also increased the proportion of bacteria containing GH 5 genes.The proportion of cellulolytic genera of Clostridium and Ruminococcus increased,whereas Bacteroides,Fibrobacter and Acetivibrio disappeared due to their inability to acclimatize to in vitro lignocellulosic conditions.The analysis of GH and 16S rRNA genes also provided a better description of cellulolytic function and phylogenetic profile.
Cellulolytic rumen bacteria Co-inoculation Methanogenic sludge Rice straw
Yuying Deng Zhenxing Huang Wenquan Ruan
School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China;Changzhou Ins School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China
国际会议
The 15th IWA World Conference on Anaerobic Digestion( 第15届IWA世界厌氧大会)
北京
英文
459-464
2017-10-17（万方平台首次上网日期，不代表论文的发表时间）