Bioelectrochemical CH4 production with antibiotics pretreatment: potential enrichment of electromethanogenesis
Methane (CH4)-producing bioelectrochemical systems (BES) are an attractive way to store excess renewable electricity and captured CO2.Studies have suggested that electromethanogenesis may play an important role in these systems.However,mechanisms and key microorganisms involved in electromethanogenesis remain unclear,primarily because of H2 produced by bacteria in the system.In an attempt to minimize biological H2 production,cathode chambers were pretreated with antibiotics (ampicillin and kanamycin) that target bacteria.We found that antibiotics pretreatment effectively reduced the proportion of H2 producing bacteria and H2-utilizing methanogens associated with the biocathode biofilm,and significantly promoted growth of methanogens from the genera Methanosarcina and Methanosaeta known to be capable of direct electron transfer.Results also showed that acetogenic activity was suppressed and that organic fermentation end-products were not present in the antibiotics-pretreated cathode chamber,suggesting that electromethanogenesis rather than acetoclastic methanogenesis could be the primary pathway for CH4 production utilized by Methanosaeta and Methanosarcina species.This shift in microbial community structure resulted in 14%-36% higher cathode capture efficiencies and more stable biocathodes.These results suggest that suppression of H2 production by antibiotics pretreatment could be a promising way to enrich for microorganisms responsible for electromethanogenesis in CH4-producing BES.
Bioelectrochemical systems Methane Biocathode Electromethanogenesis Suppression CO2 capture
H.Xu K.J.Wang Q.Yuan C.P.Wang D.E.Holmes
State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, T State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, T Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003, USA;Departmen
国际会议
The 15th IWA World Conference on Anaerobic Digestion( 第15届IWA世界厌氧大会)
北京
英文
591-592
2017-10-17（万方平台首次上网日期，不代表论文的发表时间）