Enhanced Methane Generation in Hybrid Anaerobic Reactor: Extracellular Electron Transfer pathway
In order to investigate the regulating effect of bioelectrochemistry in anaerobic digestion systems,methane-producing performance was tested in membrane-less microbial electrolysis cells (MECs) under closed-circuit (Rcc) and open-circuit (Roc) conditions using glucose as substrate.A dynamic model was built for imitating the methane production rate and microbial dynamics of functional population colonization in group RCC and Roc during start-up stage.Experiment results showed a significant improvement of methane production in RCC,the average methane production of Rcc was 0.131 m3/m3/d,which was 1.4-fold higher than that in Roc (0.055 m3/m3/d).Simulation results revealed that bioelectrochemistry has significant influence on the abundance of microorganisms involving acidogensis and methanogensis,which were verified by 16S rDNA high-through sequencing analysis.The abundance of glucose-uptaking microorganism decreased in Rcc (87% of total biomass) compared with Roc (67%),while the abundance of hydrogenotrophic methanogens increased from 8% to 14%.Electron balance analysis revealed that the alteration of acidogensis type led to more acetate and hydrogen production from glucose fermentation compared with the situation without bioelectrochemistry,and an extra expressway from acetate to hydrogen was further built by bioelectrolysis.These two effects resulted in significant enhancement of methane production in RCC.The direct contribution of bioelectrolysis process to methane production was 26% of total methane production after start-up stage.
Methane Bioelectrochemistry Dynamic simulation Microbial community
W.Z.Liu Z.C.Guo W.W.Cai A.J.Wang
Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chine State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology
国际会议
The 15th IWA World Conference on Anaerobic Digestion( 第15届IWA世界厌氧大会)
北京
英文
601-604
2017-10-17（万方平台首次上网日期，不代表论文的发表时间）