Reduction of azo dye by ANME-2d utilizing methane as the sole electron donor and carbon source
Anaerobic methane-oxidizing (AMO) microorganisms are widespread in nature and they are able to utilize sulfate,nitrate and nitrite as electron acceptors to oxidize methane.Recent studies found that some high valence metals are also reduced with methane as the sole carbon.However,there is no research about the relationship between organic contaminant degradation and methane oxidation.The aim of this study was to explore methyl orange (MO) degradation in AMO systems.It was found that the MO concentration affected its degradation efficiency.When the initial concentration was above 100 mg/L,its degradation rate was considerably inhibited.13CH4 isotope tracing experiment revealed that methane oxidation was involved in MO degradation.MO degradation products were N,N-dimethyl-p-phenylenediamine and 4-aminobenzenesulfonic acid and their stoichiometric relationship corresponded to theory.During the long-term experiment,the maximum degradation rate was 114.03 mg/L/d.After 30 d degradation,the percentage of Candidatus Methanoperedens (ANME-2d) significantly increased from 18.91% in the inoculum to 46.83%,while Candidatus Methylomirabilis (NC10) decreased from 18.67% to 4.47%.In addition,the percentage of Pseudoxanthomonas (Gammaproteobacteria) increased to 11.97% compared with 0.02% in the inoculum.Candidatus Methanoperedens likely reduced MO alone without bacterial partner utilizing methane as the sole carbon source.This work will improve the understanding of AMO microbial function and its application.
Anaerobic methane oxidation ANME-2d methyl orange biodegradation microbial community
Liang Fu Raymond J.Zeng Dandan Zhou
School of Environment, Northeast Normal University, United Kingdom CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science an
国际会议
The 15th IWA World Conference on Anaerobic Digestion( 第15届IWA世界厌氧大会)
北京
英文
644-646
2017-10-17（万方平台首次上网日期，不代表论文的发表时间）