Study on denitrifying anaerobic methane oxidation in intertidal zone
The intertidal ecosystem plays an important role in the carbon and nitrogen cycle of marine ecosystems because of great amount of organic material being decomposed by microorganisms under anaerobic conditions to produce large amounts of methane and inorganic nitrogen.As we know,the bacteria belong to the NC10 phylum (Candidatus Methylomirabilis oxyfera and Candidatus Methylomirabilis Sinica) can couple AOM to nitrite reduction through an intra-aerobic methane oxidation pathway.While the archaea belong to ANME-2d named Candidatus Methanoperedens nitroreducens can couple AOM to nitrate reduction through reverse methanogenesis pathway.In this study,the community structure,quantity and the potential methane oxidation activity of ANME-2d archaea and NC 10 phylum bacteria in the intertidal zone were detected with stable isotope tracer experiment,qPCR and high-throughput sequencing.The results showed that the potential Nr-DAMO activity and potential N-DAMO activity were: 0~1.57 and 0~1.51 nmol 13CO2 g-1 dry sediment day-1,respectively.The copy number of 16S rRNA gene of ANME-2d archaea and NC 10 phylum bacteria were 4.34× 104~1.18× 105 and 4.90× 104~3.27× 105 copies g-1 dry sediment,respectively.The contribution of DAMO process on methane removal during AOM process was as high as 65.6%~100%,which indicates that the DAMO process is an important methane sink in the intertidal ecosystem.
Denitrifying Anaerobic Methane Oxidation ANME-2d archaea NC10 phylum bacteria Intertidal Zone Greenhouse Gas
haoyang Cai Zhanfei He Hao Zhang Jiaqi Wang Jiajie Hu Ping Zheng Baolan Hu
Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China
国际会议
The 15th IWA World Conference on Anaerobic Digestion( 第15届IWA世界厌氧大会)
北京
英文
798-802
2017-10-17（万方平台首次上网日期，不代表论文的发表时间）