Experimental study on the inhibitory effect of total flavonoids of Trifolium repens L.on MCF-7 breast cancer mice
Objective to observe the inhibitory effect of total flavonoids of Trifolium repens L.on tumors in MCF-7 breast cancer mice,and to explore its mechanism and toxicity.Methods Breast cancer MCF-7 cells were subcutaneously inoculated into the right axilla to observe the effects of low,medium and high doses of total flavonoids of Trifolium repens L.on the tumor growth curve and tumor inhibition rate of tumor-bearing mice.The expression of Caspase-3 in tumor tissues was measured,and the serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),urea nitrogen(BUN),creatinine(CRE)and other biochemical indicators were detected.Results 2.5 and 5.0g/kg total flavonoids of Trifolium repens L.had significant inhibitory effects on the tumor volume and weight of McF-7 breast cancer mice.The inhibition rate of total flavonoids of Trifolium repens L.of 5.0g/kg clover reached 32.18%.The expression of Caspase-3 in subcutaneous transplanted tumor in the three dose groups was higher than that in the control group(P <0.05).The total flavonoids of Trifolium repens L.had no significant effect on serum ALT,AST,BUN and CRE.Conclusion the flavonoids can inhibit the growth of MCF-7 transplanted tumors.This effect may be related to the enhancement of Caspase-3 expression and promote the apoptosis of tumor cells.No flavonoids of Trifolium repens L.have liver and kidney toxicity in experimental mice.
Flavonoids of Trifolium repens L Breast tumor Development of Chinese herbal medicine Caspase-3
Mochang Qiu Xiyong Ye
Teaching Affairs Department of Jiangxi Medical College(Shangrao,China 334000,China Scientific Research Department of Jiangxi Medical College(Shangrao,China 334000,China
国际会议
大连
英文
221-226
2019-03-29（万方平台首次上网日期，不代表论文的发表时间）