Protective Effects of Isoliquiritigenin on Hypoxia-induced Pulmonary Artery Endothelial Cells Dysfunction
Hypoxia-induced pulmonary endothelial dysfunction plays critical roles in the pathological process of pulmonary arterial hypertension(PAH).Isoliquiritigenin(ISL),a member of the flavonoid family,possesses numerous pharmacological properties and has beneficial effects on the cardiovascular system.In this study,rat primary pulmonary artery endothelial cells(PAECs)were divided into a total 5 groups: normoxia,hypoxia,hypoxia+25μM ISL,hypoxia+50μM ISL,and hypoxia+100μM ISL.To determine the effect of ISL on the regulation of hypoxia-induced secretion of inflammatory cytokines and vasoactive factors in PAECs,the levels of ET-1,TNF-α and IL-6 were measured in cell culture supernatant.In addition,HIF-1α,ET-1,TNF-α,and IL-6 mRNA levels were also assessed.The results showed that hypoxia increased the levels of ET-1,TNF-α and IL-6 in cell culture supernatant and upregulated HIF-1α,ET-1,TNF-α and IL-6 mRNA levels in PAECs.However,ISL reduced the levels of ET-1,TNF-α and IL-6 in cell culture supernatant and prevented hypoxia-induced HIF-1α,ET-1,TNF-α and IL-6 mRNA induction.Our results showed that ISL might have protective effects on hypoxia-induced PAECs dysfunction.
Isoliquiritigenin Pulmonary arterial hypertension Hypoxia Primary pulmonary artery endothelial cells Dysfunction
Yang Jiang Yuefei Wang Yongtao Li Haifeng Jin Shanqiang Zhang Yusuan Wu Lijie Yao
Department of Anatomy,Qiqihar Medical University,Qiqihar,China Department of Physiology,Qiqihar Medical University,Qiqihar,China Department of Anatomy,Qiqihar Medical University,Qiqihar,China;Qigihar Institute of Medical and Phar
国际会议
西安
英文
89-93
2019-08-23（万方平台首次上网日期，不代表论文的发表时间）