Effects of Different Planting Methods on the Diversity of Endophytic Bacteria in Tobacco

摘要：

　　In this experiment,the tobacco variety Yunyan 87 was cultivated by 3 different planting methods.Afterwards,tobacco leaves were taken as the research material.By using the high throughput sequencing technology,DNA in the 16S rDNA-V4 region of tobacco was sequenced to analyze the abundance and diversity of species,in order to understand the diversities of endophytic bacteria in tobacco under different kinds of planting methods.The results show that the sequence of micro flora richness of 3 tobacco samples was Y6.1 > Y7.1 > Y1.1; the sequence of bacteria diversity was Y7.1 > Y6.1 > Y1.1.The bacterial diversity of Y7.1 was higher than that of Y1.1 and Y6.1.Endophytic bacteria found in samples were distributed in 13 phyla,namely Proteobacteria,Bacteroidetes,Actinobacteria,Acidobacteria,Gemmatimonadetes,Firmicutes,Chloroflexi,Planctomycetes,Nitrospirae,Verrucomicrobia,Thaumarchaeota,Thermomicrobia and Armatimonadetes.Proteobacteria and Actinobacteria were dominant bacteria in Y1.1,which accounted for 61.56%and 18.26%of the total bacteria.Proteobacteria,Acidobacteria and Actinobacteria were dominant bacteria in Y6.1,which accounted for 47.42%,19.71%and 13.40%of the total bacteria.Proteobacteria,Bacteroidetes and Actinobacteria were dominant bacteria of Y7.1,which accounted for 54.28%,15.59%and 11.24%of the total bacteria.The distribution of Morganella endophytic bacteria in Y6.1 and Y7.1 was related to the addition of biological carbon.The distribution of Fusobacterium and Proteiniclasticum endophytic bacteria in Y7.1 was related to the inter cropping with marigold.

关键词： high throughput sequencing tobacco planting method endophytic bacteria diversity

作者: Song Jin Xi Gao Zebin Chen Yuan Su Jiani Liu Lei Yu Dingkang Wang Shengguang Xu

作者单位: College of Agronomy,Kunming University,Kunming,Yunnan Province,China College of Plant Protection,Yunnan Agricultural University,Kunming,Yunnan Province,China Key Laboratory of Characteristic Biological Resource Development and Utilization of Colleges and Uni Yunnan Urban Characteristic Agriculture Engineering Technology Research Center,Kunming,Yunnan Provin Yunnan Institute of Biological Carbon Engineering Research Center,Kunming,Yunnan Province,China

会议类型: 国际会议

会议名称: 2018 7th International Conference on Medical Engineering and Biotechnology (MEDEB 2018) 2018年第七届国际医学工程与生物技术会议(MEDEB 2018)

会议地点: 南京

会议语种:英文

页码: 31-35

在线出版日期: 2018-07-14（万方平台首次上网日期，不代表论文的发表时间）