Research on Callus Promotion and Protoplast Separate of Lavender Angustifolia
To make a thorough inquiry about the optimum conditions for the separation of primary plastids from lavender-callus,providing basic data and theoretical basis for the optimization and breeding of lavender varieties.Using the lavender tender leaves as raw materials,the method of chemical induction was used to induce the dissociation of lavender to form the callus,which was used to dissociate the protoplast.The protoplast vitality was calculated with staining method.When cultured in MS medium with 2,4-D(1.8mg/L)and 6-BA(0.5mg/L),lavender callus was the highest,up to 88%; the content of callus in cellulase and pectinase were 1.8%and 0.5%under the conditions of enzymatic hydrolysis yield of protoplasts the highest,up to 1.180×105 per gram,the highest protoplast viability,induction conditions can reach 78.05%.The efficient callus induced conditions of this experiment provide data support for further study of the subsequent callus regeneration system.The protoplast isolation system was established,and the protoplast of high yield and high vitality was obtained stably.
lavender angustifolia protoplast separation tissue culture protoplast preparation
Miu Tianlin Sunyanbin Ren Guoli Xue Chunmei
Jiamusi University College of Life Sciences,Heilongjiang Province 154007,China Jiamusi University College of Science,Heilongjiang Province 154007,China
国际会议
太原
英文
369-373
2018-06-29（万方平台首次上网日期，不代表论文的发表时间）