Uptake and endosomal escape of spermine-mannan and its gene transfection on bone marrow-derived dendritic cells

摘要：

　　In a previous study, we designed a mannose receptor-specific gene delivery vehicle named spermine-mannan (SM) for macrophage mannose receptor-positive (MMR+) cells.In the present study, we confirmed the important role played by calcium in the uptake of SM/DNA complex and the endosomal escape of the complex.The result demonstrated that SM is more efficient than other vehicles are in terms of both uptake and transfection on bone marrow derived dendritic cells.This result highlights the potential applicability of SM in dendritic cell-based immunotherapy.

作者: Xin-Chi Jiang Gui-Xin Ruan Ying Hu Jian-Qing Gao

作者单位: Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou,Zhejia Department of Pharmaceutics, Zhejiang Pharmaceutical College, Ningbo, Zhejiang Province,P.R.China

会议类型: 国际会议

会议名称: The 4th Asian Symposium on Pharmaceutical Science and Technology & Enlarged Editorial Board Conference of the Asian Journal of Pharmaceutical Sciences (第四届亚洲药剂学论坛暨亚洲药物制剂科学杂志编委会扩大会议)

会议地点: 沈阳

会议语种:英文

页码: 227-228

在线出版日期: 2016-04-28（万方平台首次上网日期，不代表论文的发表时间）