Molecular weight determination of a newly synthesized guanidinylated disulfide-containing poly (amido amine) by gel permeation chromatography

摘要：

　　A cationic gene delivery vector,guanidinylated disulfide-containing poly (amido amine) (Gua-SS-PAA), was synthesized.Gel permeation chromatography (GPC) was used to evaluate the molecular weight of synthesized Gua-SS-PAA.The effects of two critical factors,namely columns and eluents, on the molecular weight measurement of Gua-SS-PAA were investigated to optimize the GPC quantitative method.The results showed that Ultrahydrogel columns (120, 250) and HAc-NaAc (0.5 M, pH 4.5) buffer solution were the optimal column and GPC eluent, respectively.The molecular weight of the synthesized Gua-SS-PAA was analyzed by the optimized GPC method and determined to be 24.66 kDa.

作者: Haonan Xing Mei Lu Lei Xian Jinmin Zhang Tianzhi Yang Pingtian Ding

作者单位: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning, 110016, China Department of Basic Pharmaceutical Sciences, School of Pharmacy, Husson University, Bangor, ME,04401

会议类型: 国际会议

会议名称: The 4th Asian Symposium on Pharmaceutical Science and Technology & Enlarged Editorial Board Conference of the Asian Journal of Pharmaceutical Sciences (第四届亚洲药剂学论坛暨亚洲药物制剂科学杂志编委会扩大会议)

会议地点: 沈阳

会议语种:英文

页码: 246-247

在线出版日期: 2016-04-28（万方平台首次上网日期，不代表论文的发表时间）