Co-existence of blaOXA-23 and blaVIM in carbapenem-resistant Acinetobacter baumannii isolates belonging to global complex 2 in a Chinese teaching hospital
Background: Carbapenem-resistant Acinetobacter baumannii (CRAB) have been a challenging concern of health-care associated infections.The aim of the current study was to investigate the molecular epidemiology and clonal dissemination of CRAB isolates in a Chinese teaching hospital.Methods: Non-duplicate clinical A.baumannii isolates were collected from inpatients, and we measured the minimal inhibitory concentrations to determine antimicrobial susceptibility.Polymerase chain reaction (PCR) and sequencing were performed to detect carbapenem-resistance genes and occurrence of transposons among CRAB isolates.Moreover, the genetic diversity among isolates and clonal dissemination were determined by repetitive element PCR-mediated DNA fingerprinting (rep-PCR) and multilocus sequence typing (MLST).Results: A total of 67 CRAB isolates displayed resistance to most of the antibiotics tested in this study, except tigecycline.We detected blaOXA-23, blaOXA-51, blaOXA-58, and blaVIM genes in 94.0%, 100.0%, 1.5%, and 80.6% of the CRAB isolates, respectively.Nevertheless, 74.6% of the CRAB isolates co-harbored the blaoxA-23 and blaVIM.Only one type of transposons was detected:Tn2008 (79.1%, 53/67).Although 12 distinctive types (A-L) were determined (primarily A type) ST195 was the most prevalent sequence type (ST).ST368, ST210, STg0, ST829, and ST136 were also detected, and all belonged to clonal complex 208 (CC208) and global complex 2 (GC2).Conclusion: The blaOXA-23 and blaVIM genes contributed to the resistance among CRAB isolates collected in our study.Notably,most of the CRAB strains co-harbored blaOXA-23 and blaVIM genes, as well as Tn2008, which could contribute to clonal dissemination.The prevalence of such organisms may underlie hospital acquired infections.
Acinetobacter baumannii blaOXA-23 and blaVIM genes rep-PCR Transposons
Zi-Yan Huang Jun Li Jian Shui Hai-Chen Wang Yong-Mei Hu Ming-Xiang Zou
Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China
国际会议
珠海
英文
1166-1172
2019-05-23（万方平台首次上网日期，不代表论文的发表时间）