Tissue-type plasminogen activator is a homeostatic regulator of synaptic function in the central nervous system
Membrane depolarization induces the release of the serine proteinase tissue-type plasminogen activator (tPA) from the presynaptic terminal of cerebral cortical neurons.Once in the synaptic cleft this tPA promotes the exocytosis and subsequent endocytic retrieval of glutamate-containing synaptic vesicles, and regulates the postsynaptic response to the presynaptic release of glutamate.Indeed, tPA has a bidirectional effect on the composition of the postsynaptic density (PSD) that does not require plasmin generation or the presynaptic release of glutamate, but varies according to the baseline level of neuronal activity.Hence,in inactive neurons tPA induces phosphorylation and accumulation in the PSD of the Ca2+/calmodulin-dependent protein kinase Ⅱα (pCaMKⅡα), followed by pCaMKⅡα-induced phosphorylation and synaptic recruitment of GluR1-containing α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors.In contrast, in active neurons with increased levels of pCaMKⅡα in the PSD tPA induces pCaMKⅡα and pGluR1 dephosphorylation and their subsequent removal from the PSD.These effects require active synaptic N-methyl-D-aspartate (NMDA) receptors and cyclin-dependent kinase 5 (Cdk5)-induced phosphorylation of the protein phosphatase 1 (PP1) at T320.These data indicate that tPA is a homeostatic regulator of the postsynaptic response of cerebral cortical neurons to the presynaptic release of glutamate via bidirectional regulation of the pCaMKⅡα/PP1 switch in the PSD.
tissue-type plasminogen activator (tPA) homeostatic plasticity Ca2+/calmodulin-dependent protein kinase post-synaptic density protein phosphatase 1 plasmin
Valerie Jeanneret Manuel Yepes
Department of Neurology & Center for Neurodegenerative Disease, Emory University School of Medicine, Department of Neurology & Center for Neurodegenerative Disease, Emory University School of Medicine,
国际会议
广州
英文
84-87
2018-07-26（万方平台首次上网日期，不代表论文的发表时间）