Study on the Anti-tumor Mechanisms of Heat Shock Protein 72/alpha-fetoprotein Epitope Peptide Complex Against Hepatocellular Carcinoma Cell Hepa1-6
Objective: Mice immunized with heat shock protein 72(HSP72)–alpha-fetoprotein(AFP)epitope peptide complex were used to study whether the complex has specific anti-tumor immune effect against AFP tumor.Methods: Kunming mice were immunized subcutaneously with HSP72-AFP peptide complex,and mice were immunized with AFP polypeptide and HSP72,respectively.The serum IFN-γ levels in mice after immunization were detected by ELISA.The killing effect of lymphocytes on hepatic cancer Hepal-6 cells was detected by MTT assay.The immune effect of protein complex was evaluated by tumor burden test in mice.Results: The serum IFN-r levels and the killing effect of lymphocytes on Hepal-6 cells in HSP72-AFP peptide complex group were significantly higher than those in AFP polypeptide and HSP72 group(P<0.01).The tumor volume in HSP72-AFP polypeptide complex group was also significantly smaller than that of the AFP polypeptide and HSP72 immunized groups(P<0.01).Conclusion: The HSP72-AFP polypeptide complex vaccine can induce tumor-specific mice to produce specific cellular immunity against AFP tumors,and its killing effect on tumor cells is significantly better than that of the single polypeptide purified vaccine.The surface HSP72-AFP polypeptide complex can induce effective anti-tumor immunity in mice.
Bing Xu Xiaoping Wang Huanping Lin
School of Shaanxi University Of Chinese Medicine,Xian,China
国际会议
上海
英文
1-5
2018-12-17（万方平台首次上网日期，不代表论文的发表时间）