Effects of Marigold Cultivation on Microbial Diversity of Tobacco-planting Soil
With Yunyan 87 as the research material,this paper used Illumina MiSeq high throughput sequencing technology to study the effect of marigold cultivation on microbial diversity of tobacco planting soil under two kinds of planting patterns.The results showed that the diversity of bacterial Alpha was represented as bacterial abundance CC3.2 > AB3.2 > BB3.2 and bacterial diversity BB3.2 > AB3.2 > CC3.2.The diversity of fungal Alpha was represented as fungi abundance BB3.2 > AB3.2 > CC3.2 and fungi diversity BB3.2>AB3.2>CC3.2.At the level of genus,the dominant bacteria of three samples were similar; three kinds of bacteria with higher abundances were Kaistorbacte,Marinobacter and Nitrososphaera.There were only small differences in the abundances of the same genus.The content of Nitrososphaera in CC3.2 was lower than that in AB3.2 and BB3.2 by 0.80%to 0.91%.The three samples had similar dominant fungi,but the abundances of fungi varied greatly.After the planting of marigold,the abundance of Aspergillus increased from the original number of 0.89%to values between 5.61%and 7.70%.Compared with single cropping of tobacco,marigold inter planting with tobacco increased the abundance by 8.6 times.The abundance of Sordariales sp rose from the original number of 3.75%to values between 4.09%and 5.03%after marigold cultivation.The abundance of Corynascella was between 6.24%and 7.39%under monoculture,and sharply decreased to 0.04%under the condition of tobacco inter planting with marigold.The decrease rate was over 99%.
high-throughput sequencing marigold cultivation tobacco-planting soil microorganism diversity
Song Jin Xi Gao Zebin Chen Yuan Su Jiani Liu Lei Yu Dingkang Wang Shengguang Xu
College of Agronomy,Kunming University,Kunming,Yunnan Province,China College of Plant Protection,Yunnan Agricultural University,Kunming,Yunnan Province,China Key Laboratory of Characteristic Biological Resource Development and Utilization of Colleges and Uni Yunnan Urban Characteristic Agriculture Engineering Technology Research Center,Kunming,Yunnan Provin Yunnan Institute of Biological Carbon Engineering Research Center,Kunming,Yunnan Province,China
国际会议
广州
英文
22-26
2018-09-01(万方平台首次上网日期,不代表论文的发表时间)