Study on the Method of Detecting Phycocyanin by Fluorescence Spectroscopy

摘要：

　　The phycocyanin is a unique protein of cyanobacteria.In this study,fluorescence spectroscopy was used to study the algae of Anabaena sinensis.Firstly,the optimal detection conditions were determined by fluorescence scanning of living algae cells: λex=620nm,λem=650nm.The relationship between the fluorescence intensity of phycocyanin and the biomass of algal cells was established(R2=0.9972).The method was applied to the study of cyanobacterial growth.The results showed that the method has high precision(RSD=3.32%)and repeats.Sex(RSD=3.87%)is good,and the sample does not need to be treated.

关键词： fluorescence spectrum phycocyanin cyanobacteria biomass

作者: Jinxia Wang Le Luo Lingling Zhan

作者单位: Chongqing Vocational Institute of Engineering,Chongqing,China

会议类型: 国际会议

会议名称: 2018 International Conference on Biomedical Engineering, Machinery and Earth Science (BEMES 2018)(2018年生物医学工程、机械与地球科学国际会议)

会议地点: 广州

会议语种:英文

页码: 72-76

在线出版日期: 2018-09-01（万方平台首次上网日期，不代表论文的发表时间）