A DNA Nano-Tweezer for Intracellular Detection of HER2 mRNA by Confocal Microscopy
DNA nanostructure plays an important role in intracellular detection strategies for its good biocompatibility and nontoxicity.Herein,we design a DNA nano-tweezer with aptamer and fluorophores to detect HER2 mRNA in living breast carcinoma cells.The designed nano-tweezer is self-assembled with ten DNA strands.It has one head and two arms with a long hangover each.A HER2 aptamer in its head is for targeting tumor cell and two overhangs with fluorophores can hybridize with HER2 mRNA.Following,the as-prepared nano-tweezer can effectively enter cancer cells by specific cross-talking between aptamers and overexpressed cell-surface receptors.Finally,the HER2 mRNA fluorescence detection is achieved by a confocal microscope,and the fluorescence intensity indicates mRNA expression quantity.Thus,this strategy provides a useful platform for efficient detection of HER2 mRNA in living cells.
HER2 mRNA DNA nano-tweezer breast carcinoma
Haiying Que Fei Mo Tong Wang Xiaoyu Yan Hongmin Ma Yurong Yan
Key Laboratory of Clinical Laboratory Diagnostics(Ministry of Education),College of Laboratory Medicine,Chongqing Medical University,Chongqing 400016,China
国际会议
广州
英文
210-214
2018-09-01（万方平台首次上网日期，不代表论文的发表时间）