INHIBITORY EFFECT AND ENZYMOLYSIS KINETICS OF LENTINAN ON Α-GLUCOSIDASE

摘要：

　　Objective: To study the inhibitory activity and enzymolysis kinetics of lentinan on α-glucosidase.Methods: The enzyme-agent screening model was used to investigate the optimal conditions of α-glucosidase action.In vitro enzyme kinetics was studied by changing the substrate concentration,reaction time,pH conditions and temperature.Results: The optimal reaction conditions of α-glucosidase were reaction time 120min,reaction temperature 50℃,buffer pH 6.0,concentration of substrate PNPG 0.1089 mol/L.Conclusion: 1g/L concentration of lentinan has good inhibitory activity against α-glucosidase,and the inhibitory effect of lentinan is competitive inhibition.

关键词： Lentinan α-glucosidase enzymolysis kinetics

作者: Meifu Wu Hongli Zhou

作者单位: School of Chemistry and Pharmaceutical Engineering,Jilin Institute of Chemical Technology,Jilin 132022,China

会议类型: 国际会议

会议名称: 2018 International Conference on New Materials and Intelligent Manufacturing (ICNMIM(2018年第一届新材料与智能制造国际会议))

会议地点: 吉林

会议语种:英文

页码: 312-314

在线出版日期: 2018-06-23（万方平台首次上网日期，不代表论文的发表时间）