Fermentative production of optically pure d-lactic acid from rice straw hydrolysate by a genetic engineered escherichia coli DX03
Lignocellulosic materials,such as rice straw,are utilised as a substrate for microbial fermentation.Rice straw was pre-treated and used for fermentative production of D-lactic acid by genetic engineered Escherichia coli DX03(ΔfrdBC ΔldhA ΔackA ΔpflB ΔpdhR::pflBp6-acEF-lpd ΔmgsA ΔadhE ΔptsG ΔmglB)in a 7 L fementer.After acid hydrolysis,detoxification and cellulase treatment,52.4 g of reducing sugars was generated from 100 g of rice straw.Following a 36 h fermentation,46.7 g of D-lactic acid was produced from the hydrolysate by DX03,achieving an optical purity of 99.8%,and a yield of 89.34%.This results suggests that E.coli strain DX03 has the potentials for fermentative production of green chemicals such as D-lactic acid,using the low-cost hydrolyzed lignocellulosic materials.
Escherichia coli DX03 Rice Straw Fermentation D-lactic acid
Jia ZHAO Hailiang PAN Xiao ZHAO Wa GAO Jinhua WANG
Key Laboratory of Fermentation Engineering,Hubei University of Technology,Ministry of EduucationWuhan 430068 China;Hubei Key Laboratoyr of industry Microbiology,Wuhan 430068 China
国际会议
武汉
英文
153-157
2018-08-21（万方平台首次上网日期，不代表论文的发表时间）