Selective binding and magnetic separation of His-tagged proteins using Fe3O4/PAM/NTA-Ni2+Magnetic Nanoparticles

摘要：

　　Fe3O4 nanoparticles coated with polyacrylamide(PAM)were synthesized.The magnetic core,with an average hydrodynamic size of 235.5 nm,allowed the magnetic nanoparticles(MNPs)rapid separation from solutions under an external magnetic field.NTA-Ni2+was modified on the surface of Fe3O4/PAM MNPs to selectively trap his-tagged green fluorescent protein(GFP).The results showed that Fe3O4/PAM/NTA-Ni2+MNPs exhibited remarkable capability of selective binding and separating his-tagged GFP.The adsorption efficiency was 93.37%.

作者: Huiling Guo Mengyun Li Shu Tu Honghao Sun

作者单位: College of Bioengineering and Food,Hunei Provincial Cooperative Innovation Center of Industrial Fermentation,Hubei University of Technology,Wuhan 430068,China

会议类型: 国际会议

会议名称: The 1st International Symposium on Application of Materials Science and Energy Materials (SAMSE 2017)

会议地点: 上海

会议语种:英文

页码: 1-6

在线出版日期: 2017-12-28（万方平台首次上网日期，不代表论文的发表时间）