Conductive Nanomaterials Induced Positive Effects on Syntrophic Methanogenesis from Butyrate Oxidation
The enhancement of anaerobic methanogenesis has been observed with supplemented conductive carbon materials,which can promote direct interspecies electron transfer(DIET)in specific co-cultures with Geobacter.However,ethanol was the sole substrate in above mentioned study,the effects of conductive nanomaterials on syntrophic oxidation of butyrate in defined co-cultures are still not evaluated.In this study,we constructed co-culture of Syntrophomonas wolfei and Methanococcus maripaludis to determine the effect of carbon nanotubes(CNTs)and reduced graphene oxide(rGO)on syntrophic butyrate oxidation and CH4 production.This investigation revealed that CH4 production was substantially accelerated when CNTs and rGO were added to the defined co-culture,respectively.No acceleration of CH4 production was observed in M.maripaludis pure-culture by adding CNTs or rGO.To better visualize the contact state between cells and conductive nanomaterials,we performed fluorescence in situ hybridization.It appeared that Syntrophomonas wolfei and Methanococcus maripaludis were intertwined in non-amended control,while CNTs or rGO seemed can be served as an electrical linkage for DIET between the two specials in nanomaterial-amended treatments.The results presented here suggest that the acceleration of syntrophic methanogenesis may be attributed to DIET in presence of CNTs or rGO,and this mechanism deserves further investigation.
syntrophic methanogenesis conductive nanomaterial butyrate
Wei Zhang Yahai Lu
College of Urban and Environmental Sciences,Peking University,Beijing 100871,China
国际会议
The 11th International Symposium on Geochemistry of the Earths Surface(GES-11)(第十一届地球表层地球化学国际研讨会)
贵阳
英文
1-4
2017-06-11（万方平台首次上网日期，不代表论文的发表时间）