Effects of Different Inoculated Pathogens on Microbial Species and Abundances of Rhizosphere Soil of Tobacco
(0) The aim of this research was to clarify the effects of different inoculated pathogens on microbial species and abundances of rhizosphere soil of tobacco.The rhizosphere soil of root-knot nematode and Phytophthora parasitica infected tobacco were collected and detected.The DNA of 16S rDNA-V4 region and ITS1 region in the rhizosphere soil of infected tobacco were sequenced through high throughput sequencing technique; the abundance and diversity of microorganisms were analyzed.In the 6S rDNA-V4 region and ITS1 region,152815 and 61505 original sequences were detected respectively.After filtering low quality sequences,147558 and 60109 effective sequences were obtained,which could be divided into 6871 and 796 OTUs.The diversity analysis showed that pathogen inoculation caused significant differentiation in the microbial community of rhizosphere soil.The Shannon index and Chao1 index significantly increased compared with the control group,indicating that pathogen inoculation could significantly increase microbial diversity and abundance.The research on microbial diversity of rhizosphere soil of tobacco after pathogen inoculation can provide reference for the development of remediation technology on soil ecology.
high throughput sequencing tobacco rhizosphere soil root-knot nematode black shank
Zebin Chen Xi Gao Li Lin Yuan Su Jiani Liu Lei Yu Dingkang Wang Shengguang Xu
College of Agronomy,Kunming University,Kunming,Yunnan Province,China College of Plant Protection,Yunnan Agricultural University,Kunming,Yunnan Province,China Key Laboratory of Characteristic Biological Resource Development and Utilization of Colleges and Uni Yunnan Urban Characteristic Agriculture Engineering Technology Research Center,Kunming,Yunnan Provin Yunnan Institute of Biological Carbon Engineering Research Center,Kunming,Yunnan Province,China
国际会议
重庆
英文
16-20
2018-11-08（万方平台首次上网日期，不代表论文的发表时间）