Genome sequence and comparative analysis of a Vibrio cholerae O139 strain E306 isolated from a cholera case in China
Background: Vibrio cholerae is a human intestinal pathogen and V.cholerae of the O139 serogroups are responsible for the current epidemic cholera in China.In this work, we reported the whole genome sequencing of a V.cholerae O139 strain E306 isolated from a cholera patient in the 306th Hospital of PLA, Beijing, China.Results: We obtained the draft genome of V.cholerae O139 strain E306 with a length of 4,161,908 bps and mean G + C content of 47.7%.Phylogenetic analysis indicated that strain E306 was very close to another O139 strain, V.cholerae MO10, which was isolated during the cholera outbreak in India and Bangladesh.However, unlike MO10, strain E306 harbors the El Tor-specific RS1 element with no pre-CTX prophage (VSK), very similar to those found in some V.cholerae O1 strains.In addition, strain E306 contains a SXT/R391 family integrative conjugative element (ICE) similar to ICEVchlnd4 and SXT MO10, and it carries more antibiotic resistance genes than other closest neighbors.Conclusions: The genome sequence of the V.cholerae O139 strain E306 and its comparative analysis with other V.cholerae strains we present here will provide important information for a better understanding of the pathogenicity of V.cholerae and their molecular mechanisms to adapt different environments.
Cholera toxin prophage Integrative conjugative elements Antibiotic resistance genes
Yong Yi Na Lu Fei Liu Jing Li Ruifen Zhang Liping Jia Hua Jing Hu Xia Yi Yang Baoli Zhu Yongfei Hu Yan Cui
The 306th Hospital of PLA, Beijing 100101, China CAS key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology,Chinese Acad Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical
国际会议
北京
英文
73-79
2018-03-29（万方平台首次上网日期，不代表论文的发表时间）