Diversity of the Tetracycline Mobilome within a Chinese Pig Manure Sample
Tetracycline antibiotics are widely used in livestock, and tetracycline resistance genes (TRG) are frequently reported in the manure of farmed animals.However, the diversity of TRG-carrying transposons in manure has still been rarely investigated.Using a culture-free functional metagenomic procedure, combined with large-insert library construction and sequencing, bioinformatic analyses, and functional experiments, we identified 17 distinct TRGs in a single pig manure sample, including two new tet genes: tet(59), encoding a tetracycline efflux pump, and tet(W/N/W), encoding mosaic ribosomal protection.Our study also revealed six new TRG-carrying putative nonconjugative transposons: Tn5706-1ike transposon Tn6298, IS200/605-related transposon Tn6303, Tn3 family transposon Tn6299, and three ISCR2-related transposons, Tn62300, Tn62301, and Tn62302.
Sébastien Oliviet Leclercq Chao Wang Yaxin Zhu Hai Wu Xiaochen Du Zhipei Liu Jie Feng
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, College of Life Sciences, Hebei University,Baoding, China
国际会议
北京
英文
147-155
2018-03-29（万方平台首次上网日期，不代表论文的发表时间）