Genomic sequence based scanning for drug resistance-associated mutations and evolutionary analysis of multidrug-resistant and extensively drug-resistant Mycobacterium tuberculosis
Objective: To better understand the molecular mechanisms and evolution of drug resistance in Mycobacterium tuberculosis (M.tuberculosis), we performed a genomic sequence based scanning of drug resistance-associated loci for multidrug-resistant (MDR) and extensively drug-resistant (XDR) M.tuberculosis strains.Materials and methods: Forty-five pairs of primers covering known drug resistance-associated loci compiled in the TBDReaMDB database were designed to perform the analysis of drug resistance-associated mutations for 14 M.tuberculosis clinical isolates from TB patients in China.Genetic diversity and evolutionary analysis was done using concatenated nucleotide sequences of drug resistance-associated loci.Results: Forty-four types of mutations were identified in 14 M.tuberculosis clinical isolates.Average nucleotide diversity for drug resistance-associated loci increased in the M.tuberculosis isolates as the drug resistance increased (π =0, π =0.00021, and π =0.00028 for susceptible, MDR, and XDR isolates, respectively).The dN/dS ratios for coding regions of drug resistance-associated genes in MDR and XDR isolates were 2.73 and 1.83, respectively.MDR and XDR isolates were distributed sporadically on different branches in the phylogenetic trees.
Multidrug-resistant tuberculosis (MDR-TB) Extensively drug-resistant tuberculosis(XDR-TB) Drug susceptibility test(DST) Evolutionary analysis
Cui Hua Liu Hong Min Li Na Lu Qi Wang Yong Liang Hu Xi Yang Yong Fei Hu Patrick C.Y.Woo George F.Gao Baoli Zhu
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Aca Institute for Tuberculosis Research, The 309 Hospital, Beijing, China State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of
国际会议
北京
英文
327-337
2018-03-29（万方平台首次上网日期，不代表论文的发表时间）