Analysis of Drug Resistance Determinants in Klebsiella pneumoniae Isolates from a Tertiary-Care Hospital in Beijing, China
Background:The rates of multidrug-resistant (MDR), extensively drug-resistant (XDR) and pandrug-resistant (PDR) isolates among Enterobacteriaceae isolates, particularly Klebsiella pneumoniae, have risen substantially worldwide.Methodology/Principal Findings:To better understand the molecular mechanisms of drug resistance in K.pneumoniae, we analyzed the drug resistance determinants for K.pneumoniae isolates co ected from the 306 Hospital, a tertiary-care hospital in Beijing, China, for the period of September 1, 2010-October 3t, 2011.Drug susceptibility testing, PCR amplification and sequencing of the drug resistance determinants were perforrned.Conjugation experiments were conducted to examine the natural ability of drug resistance to disseminate among Enterobacteriaceae strains using a sodium azide-resistant Escherichia coli JS3 strain as a recipient.Among the 223 consecutive non-repetitive K.pneumoniae isolates included in this study, 101 (45.3%) were extended-spectrum beta-lactamases (ESBLs) positive.The rates of MDR,XDR, and PDR isolates were 61.4% (n =137), 22.0% (n =49), and 1.8% (n=4), respectively.Among the tested drug resistance-associated genes, the following ones were detected at relatively high rates blaCTX-M-10 (80, 35.9%), aacC2 (73, 32.7%), dhfr (62, 27.8%), qnrS (58, 26.0%), aacA4 (57, 25.6%), aadA1 (56, 25.1%).Results from conjugation experiments indicate that many of the drug resistance genes were transmissible.Conclusions/Significance:Our data give a snapshot of the complex genetic background responsible for drug resistance in K.pneumoniae in China and demonstrate that a high degree of awareness and monitoring of those drug resistance determinants are urgently needed in order to better control the emergence and transmission of drug-resistant K.pneumoniae isolates in hospital settings.
Bin Li Yong Yi Qi Wang Patrick C.Y.Woo Lin Tan Hua Jing George F.Gao Cui Hua Liu
Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of M The 306 Hospital, Beijing, China Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of M State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of
国际会议
北京
英文
346-357
2018-03-29（万方平台首次上网日期，不代表论文的发表时间）