Genotypic Analysis of Klebsiella pneumoniae Isolates in a Beijing Hospital Reveals High Genetic Diversity and Clonal Population Structure of Drug-Resistant Isolates
Background: The genetic diversity and the clinical relevance of the drug-resistant Klebsiella pneumoniae isolates from hospital settings are largely unknown.We thus conducted this prospective study to analyze the molecular epidemiology of K.pneumoniae isolates from patients being treated in the 306 Hospital in Beijing, China for the period of November 1, 2010-October 31, 2011.Methodology/Principal Findings: Antibiotic susceptibility testing, PCR amplification and sequencing of the drug resistance-associated genes,and multilocus sequence typing (MLST) were conducted.A total of 163 isolates were analyzed.The percentage of MDR, XDR and PDR isolates were 63.8% (104), 20.9 (34), and 1.8% (3), respectively.MLST results showed that 60 sequence types (STs) were identified, which were further separated by eBURST into 13 clonal complexes and 18 singletons.The most dominant ST was ST1S (10.4%).Seven new alleles and 24 new STs were first identified in this study.Multiple logistic regression analysis revealed that certain clinical characteristics were associated with those prevalent STs such as: from ICU, from medical ward, from community acquired infection, from patients without heart disease, from patients with treatment success, susceptible to extended spectrum cephalosporin, susceptible to cephamycins, susceptible to fluoroquinolones, and with MDR.Conclusions/Significance: Our data indicate that certain drug-resistant K.pneumoniae clones are highly prevalent and are associated with certain clinical characteristics in hospital settings.Our study provides evidence demonstrating that intensive nosocomial infection control measures are urgently needed.
Qi Wang Bin Li Alan K.L.Tsang Yong Yi Patrick C.Y.Woo Cui Hua Liu
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Aca State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of The 306 Hospital, Beijing,China
国际会议
北京
英文
358-369
2018-03-29（万方平台首次上网日期，不代表论文的发表时间）