Structural Diversity of Class 1 Integrons and Their Associated Gene Cassettes in Klebsiella pneumoniae Isolates from a Hospital in China
Background: Klebsiella pneumoniae strains carrying class 1 integrons are becoming more common worldwide and their role in the dissemination of drug resistance is significant.The aim of this study was to characterize the structural diversity of class 1 integrons and their associated gene cassettes in K.pneumoniae isolates from hospital settings.Methodology/Principal Findings: We analyzed a total of 176 K.pneumoniae isolates in a tertiary-carehospital in Beijing, China for the period of November 1, 2010-October 31, 2011.The presence of class 1 integrons and gene cassettes was analyzed by PCR and sequencing.The prevalence of class 1 integrons was 51.1% (90/176).Fourteen different gene cassettes and 10 different gene cassette arrays were detected, dfrA and aadA cassettes were predominant and cassette combination dfrA1-orfC was most frequently found (13.6%, 24/176).Strong association between resistance to a variety of drugs (both phenotypes and the associated geneS)and the presence of class 1 integrons was observed.In addition, we also identified an association between some previously identified prevalent sequence types (such as ST11, ST15, ST147, ST562, and ST716) and the presence of class 1 integrons.Conclusions/Significance: Data from this study demonstrated that class 1 integrons are highly diverse and are associated with a variety of drug resistance phenotypes, drug resistance genes, as well as genotypes among K.pneumoniae isolates.Continuous monitoring of gene cassettes in class 1 integrons is warranted to improve the understanding and control of drug resistance among hospital settings.
Bin Li Yongfei Hu Qi Wang Yong Yi Patrick C.Y.Woo Hua Jing Baoli Zhu Cui Hua Liu
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Aca The 306 Hospital, Beijing, China State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of
国际会议
北京
英文
370-378
2018-03-29（万方平台首次上网日期，不代表论文的发表时间）