Genomic and transcriptomic analysis of NDM-1 Klebsiella pneumoniae in spaceflight reveal mechanisms underlying environmental adaptability
The emergence and rapid spread of New Delhi Metallo-beta-lactamase-1 (NDM-1)-producing Klebsiella pneumoniae strains has caused a great concern worldwide.To better understand the mechanisms underlying environmental adaptation of those highly drug-resistant K.pneumoniae strains, we took advantage of the Chinas Shenzhou 10 spacecraft mission to conduct comparative genomic and transcriptomic analysis of a NDM-1 K.pneumoniae strain (ATCC BAA-2146) being cultivated under different conditions.The samples were recovered from semisolid medium placed on the ground (D strain),in simulated space condition (M strain), or in Shenzhou 10 spacecraft (T strain) for analysis.Our data revealed multiple variations underlying pathogen adaptation into different environments in terms of changes in morphology, H2O2 tolerance and biofilm formation ability, genomic stability and regulation of metabolic pathways.Additionally, we found a few non-coding RNAs to be differentially regulated.The results are helpful for better understanding the adaptive mechanisms of drug-resistant bacterial pathogens.
Jia Li Fei Liu Qi Wang Pupu Ge Patrick C.Y.Woo Jinghua Yan Yanlin Zhao George F.Gao Cui Hua Liu Changting Liu
Nanlou Respiratory Diseases Department, Chinese PLA General Hospital, No.28 Fuxing Road, Haidian Dis CAS key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Aca State Key Laboratory of Emerging Infectious Diseases,Department of Microbiology, The University of H National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prev Nanlou Respiratory Diseases Department, Chinese PLA General Hospital, No.28 Fuxing Road, Haidian Dis
国际会议
北京
英文
404-414
2018-03-29（万方平台首次上网日期，不代表论文的发表时间）