Synthesis of multiple Pseudomonas aeruginosa biofilm matrix exopolysaccharides is post-transcriptionally regulated
Exopolysaccharide is a critical biofilm matrix component, yet little is known about how the synthesis of multiple exopolysaccharides Is regulated.Pseudomonas aeruginosa can produce several biofilm matrix exopolysaccharides that include alginate, Psl and Pel.Here we demonstrated that AlgC, a key enzyme that provides sugar precursors for the synthesis of alginate and lipopolysaccharides (LPS) is also required for both Psl and Pel production.We showed that forced-synthesis of Psl in alginate-producing mucoid bacteria reduced alginate production but this was not due to transcription of the alginate biosynthesis-operon.Likewise, when either alginate or Psl were overproduced, levels of B-band LPS decreased.Induction of Pel resulted in a reduction of Psl levels.Because the effects of reduced exopolysaccharide synthesis when another is overproduced didnt appear to be regulated at the transcriptional level, this suggests that the biosynthesis pathways of Psl, Pel, alginate, and LPS compete for common sugar precursors.As AlgC is the only enzyme that provides precursors for each of these exopolysaccharides, we propose that AlgC is a key checkpoint enzyme that coordinates the total amount of exopolysaccharide biosynthesis by controlling sugar precursor pool.Our data also provide a plausible strategy that P.aeruginosa utilizes to modulate its biofilm matrix exopolysaccharides.
Luyan Ma Juan Wang Shiwei Wang Erin M.Anderson Joseph S.Lam Matthew R.Paresk Daniel J.Wozniak
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Department of Molecular and Cellular Biology,University of Guelph, Guelph, Ontario, Canada Department of Microbiology, University of Washington,Seattle, WA, USA Department of Microbial Infection and Immunity,Department of Microbiology, Center for Microbial Inte
国际会议
北京
英文
557-567
2018-03-29（万方平台首次上网日期，不代表论文的发表时间）