The roles of biofilm matrix polysaccharide Psl in mucoid Pseudomonas aeruginosa biofilms
The opportunistic pathogen Pseudomonas aeruginosa causes life-threatening, persistent infections in patients with cystic fibrosis (CF).Persistence is attributed to the ability of these bacteria to form structured communities (biofilms).Biofilms rely on an extracellular polymeric substances matrix to maintain structure.Psl exopolysaccharide is a key matrix component of nonmucoid biofilms, yet the role of Psl in mucoid biofilms is unknown.In this report, using a variety of mutants in a mucoid P.aeruginosa background, we found that deletion of Psl-encoding genes dramatically decreased their biofilm formation ability, indicating that Psl is also a critical matrix component of mucoid biofilms.Our data also suggest that the overproduction of alginate leads to mucoid biofilms, which occupy more space, whereas Psl-dependent biofilms are densely packed.These data suggest that Psl polysaccharide may have significant contributions in biofilm persistence in patients with CF and may be helpful for designing therapies for P.aeruginosa CF infection.
Psl polysaccharide mucoid biofilm Pseudomonas aeruginosa cystic fibrosis
Luyan Ma Shiwei Wang Di Wang Matthew R.Parsek Daniel J.Wozniak
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Department of Microbiology, University of Washington, Seattle, WA, USA Division of Infectious Disease, Department of Microbiology, Center for Microbial Interface Biology,
国际会议
北京
英文
579-582
2018-03-29（万方平台首次上网日期，不代表论文的发表时间）