Processivity of endoglucanase EG1 from volvariella volvacea
Phosphoric acid swollen cellulose(PASC)was hydrolyzed by EG1(CD)which is an endoglucanase lacks cellulose binding domain from volvarilla volvacea.It was observedthat a part of mass(6.5%in 50 hrs)transfer into soluble phase,and based on BCA method,the ratio of soluble reducing groups(SRGs)to insoluble reducinggroups(IRGs)was 1.7to 1.3 depend on the incubation timerange from 1-50 hrs.Furthermore,a modified bicinchoninic acid(BCA)methoud was usedto determine the number of SRGsand sensitive fluorescence measurementwas used to quantification of theend-labeled soluble reducing group(EL)which is generated byexo-initiation and IRGs generated by endo and reducing endexo-initiations,and theapparent processivity(Papp)and the processivity initiated by exo-mode(Pexo)was calculated.The results indicated that Pappof EG1(CD)is 1.74 andsimilar to the ratio of SRGs to IRGs which was measured by BCA method.Pexo is approximately 3.2%of Papp and it is muchhigher than that of the literature cite.
Yuzhi Bu Jun Wang Shufang Wu
Jiangsu Provincial Key Laboratory of Pulp and Paper Science and Technology,Nanjing Forestry University Nanjing,210037,China
国际会议
第五届制浆造纸新技术国际研讨会(ISETPP)暨第三届国际造纸与环境学术大会(IPEC)
广州
英文
1-4
2016-11-07（万方平台首次上网日期，不代表论文的发表时间）