PRODUCTION OF ALKALI-TOLERANT ENDOGLUCANASE BY HOMOLOGOUS OVEREXPRESSIO OF GENE EGLS IN BACILLUS SUBTILIS-Y106 AND APPLICATION IN IMPROVEMENT OF PULP PROPERTIES

摘要：

　　An endoglucanase gene eglS,was homologous overexpressed in Bacillus subtilis-Y106 to enhance the endoglucanase production.The resulting CMCase activity of crude enzyme from the liquor fermentation of the engineered strain was about 10.2 IU/mL at 36 h of fermentation,which was 10 times higher than that of the native Bacillus subtilis-Y106.Molecular weight,optimal temperature and pH of the endoglucanase EglS were detected respectively.The enzyme activity of 85%could be maintained at pH 8.5.Potential of the endoglucanase EglS in improvement of wheat straw pulp was also evaluated.

关键词： Alkali-tolerant Endoglucanase Homologous overexpression Enzyme properties Pulp improvement

作者: Meimei Wang Yanxin Ye Daolei zhang Jian Du Jian Zhao

作者单位: State Key Laboratory of Microbial Technology,Shandong University,250100,China

会议类型: 国际会议

会议名称: 第五届制浆造纸新技术国际研讨会(ISETPP)暨第三届国际造纸与环境学术大会(IPEC)

会议地点: 广州

会议语种:英文

页码: 1-4

在线出版日期: 2016-11-07（万方平台首次上网日期，不代表论文的发表时间）