UNDERSTANDING THE EFFECT OF SUBSTRATE LIGNIN ON CELLULASE ADSORPTION AND ENZYMATICHYDROLYSIS USING QCM ANALYSIS
Lignin has been considered as an inhibition in the enzymatic hydrolysis of lignocellulosic biomasses.For understanding the effect of substrate lignin on cellulase adsorption and enzymatic hydrolysis,commercial microcrystal cellulose,isolated milled-wood lignin from green liquor pretreated(GL-MWL)and untreated poplar(MWL)were dissolved in LiCl/DMSO solvent system to prepare the ultrathin cellulose,lignin and bicomponent films on gold supports by spin-coating.The quartz crystal microblalnce(QCM)was used to monitor the cellulase adsorption and the cellulolytic reactions on the surface of the films.The behaviors of cellulase binding and substrate hydrolysis were analyzed with kinetic models.QCM analysis provided a direct evidence on the irreversible adsorption of cellulase on substrate lignin.Compared with MWL,GL-MWL showed higher affinity with cellulase.Cellulase adsorption on substrate increased and enzymatic hydrolysis hindered with the increase of lignin content in bicomponent films.However,it is very interesting that the existence of lignin resulted in a high initial hydrolysis rate,though the final sugar conversion decreased with the lignin content.
milled-wood lignin (MWL) quartz crystal microblance (QCM) cellulase adsorption enzymatic hydrolysis bicomponent film
Tingyue Cao Chong Jiang Bo Jiang Wenjuan Wu Yongcan Jin
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,Nanjing Forestry University,Nanjing,210037,China
国际会议
第五届制浆造纸新技术国际研讨会(ISETPP)暨第三届国际造纸与环境学术大会(IPEC)
广州
英文
1-4
2016-11-07（万方平台首次上网日期，不代表论文的发表时间）