INTERACTIONS BETWEEN ENZYMES AND CELLULOSE MODEL FILMS UNVEILED BY QCM-D TECHNIQUE
To understand the mechanism of cellulose hydrolysis and super strength of protein-cellulose hybrid composites,it is necessary to study the interactions between enzymes and cellulose substrates.In this investigation,impacts of substrate polymorphs on the adsorption of cellulases were investigated by QCM-D technique.Initially,cellulose model films with allomorphs of Ⅰ,Ⅱ and the hybrid were prepared.It followed the adsorption of Trichoderma reesei on model cellulose films monitored by QCM-D technique.The results showed that cellulose nanocrystal with allomorph Ⅱ(CNC-Ⅱ)had the lowest adsorption capacity with the total amount of 179.20 ng·cm-2,but the highest reversible binding ratio of 33.72%.Whereas CNC-Ⅱ presented the fastest enzymatic hydrolysis.Whats more,the conformation of adsorbed enzyme layer on CNC-Ⅱ substrate was softer and looser than those on CNC-Ⅰ and the hybrid substrates.The results indicated that the polymorphism of cellulose has a very important impact on the reversible adsorbed amount of enzymes and the conformation of adsorbed enzyme layers on cellulose substrate; it is maybe the reason why it has the greatest effect on hydrolysis speed,if compared with enzyme adsorption amount and substrates crystallinity degree.
Ersuo Jin Fanghui Hu Yu Zhang Fang Yang Junlong Song
Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology,Nanjing Forestry University,Nanjing 210037,Jiangsu,Peoples Republic of China
国际会议
第五届制浆造纸新技术国际研讨会(ISETPP)暨第三届国际造纸与环境学术大会(IPEC)
广州
英文
1-5
2016-11-07（万方平台首次上网日期，不代表论文的发表时间）