FORMATION KINETICS OF SELF-ASSEMBLING NANOFIBERS FORM OXIDIZED SILK FIBROIN
Biomaterials derived from Bombyx mori silk fibroin(SF)represent promising candidates for tissue regeneration due to their mechanical properties,processing plasticity,and low inflammatory and immunogenic responses.To produce biocompatible,mechanically robust and conductive materials,chemical oxidation using sodium hyprochlorite(NaClO)was utilized to introduce carboxyl groups onto silk fibroin.When NaClO addition was below 5 mM/g SF,the carboxyl content increased almost linearly.A final carboxyl content of 1.09 mM/g SF was obtained at 2 mmol/g NaClO added,corresponding to~47%of the primary hydroxymethyl groups on the silk.After purification,the weight remaining of oxidized silk(OxSF)was about 87%.However,the SF protein maybe degraded when excess amounts of NaClO were added(over 5 mmol/g SF).Interestingly,both infrared(IR)spectroscopy and circular dichroism(CD)spectra demonstrated that the resulting OxSF self-assembled into β-sheet structures in aqueous conditions and this change in the kinetics of β-sheet formation was likely due to the carboxylation of the protein.The self-assembly of the OxSF resulted nanofibers in neutral aqueous solution,which occurred at room temperature in 2 days.Thereafter,the formation mechanism of OxSF nanofibers was elucidated from morphological observations using TEM as well as the kinetic studies by Thioflavin T fluorescence methods.The length of the resulted nanofibers was>1 μm with the average width of 20 nm.
Ke Zheng Jie Jiang Liang Liu Zhiguo Wang Yimin Fan
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,Jiangsu Key Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,Jiangsu Pro
国际会议
第五届制浆造纸新技术国际研讨会(ISETPP)暨第三届国际造纸与环境学术大会(IPEC)
广州
英文
1-5
2016-11-07（万方平台首次上网日期，不代表论文的发表时间）